Antimicrobial effect of Damask rose extracts
Researchers have studied the effects of aqueous, ethanolic and Estonian extracts of Damask rose on a specific type of bacterium that causes nosocomial infections.
Nowadays, due to the increasing resistance of bacteria to antibiotics derived from microorganisms, the use of antimicrobial compounds in plants as natural compounds that have lethal and inhibitory effects on pathogens has received more attention. Escherichia coli is one of the most important bacteria causing nosocomial infections, and researchers have studied the antimicrobial effect of various Damask rose extracts on Escherichia coli due to the predominant resistance of these bacteria to a wide range of antibiotics.
In this study by Sepideh Gholami, Faculty Member of Operating Room, Khorasan University of Medical Sciences, Hedyeh Rahim Pourjahani, Student Research Committee of Khorasan University of Medical Sciences, Neda Bakhshabadi, Master of Chemical Engineering, Quchan Azad University and Reza Besharti, Faculty Member of Microbiology This experimental-laboratory study was performed in the research laboratory of North Khorasan University of Medical Sciences, said North Khorasan University of Medical Sciences.
All of the culture media used in this study included Mueller Hinton Agar and soy broth (from Merck, Germany), the authors write. The microorganisms used in this study were purchased from the collection of bacteria and industrial fungi in Iran. The shoots of Damask rose were collected from North Khorasan in spring and after drying in the shade, they were prepared in powder form and used for experiments.
The researchers used the soaking method to prepare the extract. They soaked 30 g of the sample in 270 ml of water, 96% ethanol, and acetone for 24 hours, incubated at 37 ° C, and centrifuged after this time. The researchers determined the minimum inhibitory concentration of Damask rose after repeating the experiments three times.
The results of this study showed that ethanolic extract has a greater antimicrobial effect than other extracts, the study said. The diameter of the halo and the minimum inhibitory concentration for Damask rose ethanolic extract are 21 mm and 25 mg / ml, respectively. Damask rose aqueous extract had no antimicrobial properties compared to the studied bacteria.
This article describes the antimicrobial effect of Damask rose aqueous, ethanolic and Estonian extracts: The antimicrobial effect of Damask rose extracts was investigated by both disk diffusion and liquid dilution methods. The liquid dilution method is a very sensitive and complex method. In this method, different components of the extract are in direct contact with microorganisms and there is no problem of penetration of the compound into the culture medium, so that if the extract has a slight dissolution in water, still due to direct contact with microorganisms, the ability to affect it. It has.
The researchers concluded from various experiments performed in this study, considering the antimicrobial properties of Damask rose ethanolic extract in this study, that it can be used as a suitable alternative to antibiotics in the treatment of infections by conducting further research.